大鼠促肾上皮质激素释放激素(CRH)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat CRH(Corticotropin Releasing Hormone) ELISA Kit","alternative_names":"CRF; Corticotropin Releasing Factor; Corticoliberin","species":"Rat","assay_type":"Competitive Inhibition","sensitivity":"0.14 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2h","research_area":"Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01143","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10459-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10459-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10459",}