人Ⅱ型胶原(COL2)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human COL2(Collagen Type II) Microsample ELISA Kit","alternative_names":"Collagen Type 2; Col 2; Collagen Type II; Col II","species":"Human","assay_type":"Sandwich","sensitivity":"0.24 ng\/mL","standard":"50 ng\/mL","detection_range":"0.78-50 ng\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Developmental science;Hepatology;Bone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL2 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10464MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10464MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10464MS",}