小鼠甘露聚糖结合凝集素关联丝氨酸蛋白酶1(MASP1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse MASP1(Mannose Associated Serine Protease 1) ELISA Kit","alternative_names":"CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5","species":"Mouse","assay_type":"Sandwich","sensitivity":"6.7 ng\/mL","standard":"1000 ng\/mL","detection_range":"15.63-1000 ng\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;Metabolic pathway;Apoptosis;Infection immunity;Hematology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P98064","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10478-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10478-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10478",}