小鼠色素上皮衍生因子(PEDF)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse PEDF(Pigment Epithelium Derived Factor) Microsample ELISA Kit","alternative_names":"SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.056 µg\/mL","standard":"10 µg\/mL","detection_range":"0.16-10 µg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PEDF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PEDF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PEDF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PEDF in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P97298","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10493MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10493MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10493MS",}