小鼠血管内皮钙黏蛋白(VE-Cadherin)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit","alternative_names":"CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5","species":"Mouse","assay_type":"Sandwich","sensitivity":"1.21 ng\/mL","standard":"200 ng\/mL","detection_range":"3.13-200 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"CD & Adhesion molecule;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P55284","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10552-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10552-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10552",}