小鼠b2微球蛋白(BMG/b2-MG)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse BMG\/b2-MG(Beta-2-Microglobulin) ELISA Kit","alternative_names":"Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.11 µg\/mL","standard":"20 µg\/mL","detection_range":"0.32-20 µg\/mL","sample_type":"serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Kidney biomarker;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMG\/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMG\/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMG\/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMG\/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01887","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10625-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10625-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10625",}