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牛乳铁传递蛋白(LTF)酶联免疫吸附检测试剂盒

  • 货号:ELK10662
  • 价格:¥2,800.00 元
  • 点击:1935
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产品介绍

产品信息

英文名称Cattle LTF(Lactoferrin) ELISA Kit
别名HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Cattle
检测类型Sandwich
灵敏度17.6 ng/mL
检测范围78.13-5000 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, saliva, milk,cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Tumor immunity;Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P24627

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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