大鼠可溶性末端补体复合物sC5b-9(sC5b-9)酶联免疫吸附检测试剂盒
- 货号：ELK10683
- 价格：￥2,800.00 元
- 点击：1285
产品介绍
产品信息
| 英文名称 | Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit |
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| 别名 | MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9 |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | Rat |
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| 检测类型 | Sandwich |
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| 灵敏度 | 13.1 ng/mL |
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| 检测范围 | 31.25-2000 ng/mL |
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| 样本类型 | serum, plasma and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Signal transduction;Metabolic pathway;Apoptosis;Infection immunity; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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