小鼠补体因子B(CFB)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse CFB(Complement Factor B) Microsample ELISA Kit","alternative_names":"CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3\/C5 convertase; Glycine-rich beta glycoprotein","species":"Mouse","assay_type":"Sandwich","sensitivity":"2.49 µg\/mL","standard":"400 µg\/mL","detection_range":"6.25-400 µg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Immune molecule;Hematology;Autoimmunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFB in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P04186","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10687MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10687MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10687MS",}