牛胰岛素样生长因子结合蛋白4(IGFBP4)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit","alternative_names":"BP4; HT29-IGFBP; IBP4","species":"Cattle","assay_type":"Competitive Inhibition","sensitivity":"0.56 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"2h","research_area":"Apoptosis;Tumor immunity;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q05716","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10693-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10693-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10693",}