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人S100钙结合蛋白A12(S100A12)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK1073MS
  • 价格:¥2,400.00 元
  • 点击:1018

产品介绍

产品信息

英文名称Human S100A12(S100 Calcium Binding Protein A12) Microsample ELISA Kit
别名CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Human
检测类型Sandwich
灵敏度30 pg/mL
检测范围78.13-5000 pg/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;Infection immunity;Immune molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P80511

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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