小鼠中脑星形胶质细胞来源神经营养因子(MANF)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse MANF(Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit","alternative_names":"ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.047 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MANF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MANF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MANF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MANF in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9CXI5","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10820-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10820-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10820",}