斑马鱼类雌二醇(E2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Zebrafish E2(Estradiol) ELISA Kit","alternative_names":"17B-Estradiol; Oestradiol; Beta-Estradiol","species":"Zebrafish","assay_type":"Competitive Inhibition","sensitivity":"12.46 pg\/mL","standard":"3000 pg\/mL","detection_range":"46.88-3000 pg\/mL","sample_type":"serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Endocrinology;Reproductive science;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish E2 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK10939-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK10939-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK10939",}