人可溶性末端补体复合物sC5b-9(sC5b-9)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human sC5b-9(Soluble Terminal Complement Complex C5b-9) Microsample ELISA Kit","alternative_names":"MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9","species":"Human","assay_type":"Sandwich","sensitivity":"19.9 ng\/mL","standard":"5000 ng\/mL","detection_range":"78.13-5000 ng\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11117MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11117MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11117MS",}