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大鼠玻连蛋白(VTN)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK11167MS
  • 价格:¥2,800.00 元
  • 点击:2605
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产品介绍

产品信息

英文名称Rat VTN(Vitronectin) Microsample ELISA Kit
别名V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.85 µg/mL
检测范围3.13-200 µg/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;Metabolic pathway;Tumor immunity;Hepatology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VTN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q3KR94

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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