小鼠磷脂酰肌醇甘油生物合成类F蛋白(PIGF)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit","alternative_names":"PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1","species":"Mouse","assay_type":"Competitive Inhibition","sensitivity":"6.3 ng\/mL","standard":"1000 ng\/mL","detection_range":"15.6-1000 ng\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"2h","research_area":"Signal transduction;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11219-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11219-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11219",}