大鼠糖类抗原15-3(CA15-3)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat CA15-3(Carbonhydrate Antigen 15-3) Microsample ELISA Kit","alternative_names":"CA15-3","species":"Rat","assay_type":"Sandwich","sensitivity":"3.74 pg\/mL","standard":"500 pg\/mL","detection_range":"7.82-500 pg\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11235MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11235MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11235MS",}