人HLAⅡ类组织相容性抗原,DRα链(HLA-DRA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit","alternative_names":"MHC class II antigen DRA;HLA-DRA;HLA-DRA1","species":"Human","assay_type":"Sandwich","sensitivity":"0.053 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Immunology","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-DRA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-DRA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-DRA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-DRA in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01903","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11289-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11289-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11289",}