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人真核翻译起始因子4E结合蛋白1(EIF4EBP1)酶联免疫吸附检测试剂盒

  • 货号:ELK11293
  • 价格:¥2,800.00 元
  • 点击:2686

产品介绍

产品信息

英文名称Human EIF4EBP1(Eukaryotic Translation Initiation Factor 4E Binding Protein 1) ELISA Kit
别名Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1; eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I);EIF4EBP1

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Human
检测类型Sandwich
灵敏度0.051 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF4EBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF4EBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF4EBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF4EBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q13541

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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