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人胰岛素样生长因子结合蛋白3(IGFBP3)酶联免疫吸附检测试剂盒

  • 货号:ELK1139
  • 价格:¥2,400.00 元
  • 点击:2573
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产品介绍

产品信息

英文名称Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
别名BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Human
检测类型Sandwich
灵敏度0.068 ng/mL
检测范围0.16-10 ng/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长3.5h

研究信息

研究领域Metabolic pathway;Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P17936

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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