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斑马鱼氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒

  • 货号:ELK11395
  • 价格:¥2,800.00 元
  • 点击:1207

产品介绍

产品信息

英文名称Zebrafish NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
别名NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Zebrafish
检测类型Sandwich
灵敏度15.1 pg/mL
检测范围39.07-2500 pg/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Endocrinology;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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