猪食欲素(OX)酶联免疫吸附检测试剂盒

英文名称	Pig OX(Orexin) ELISA Kit
别名	HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor

种属	Pig
检测类型	Competitive Inhibition
灵敏度	4.53 pg/mL
检测范围	15.63-1000 pg/mL
样本类型	serum, plasma, tissue homogenates and other biological fluids
实验时长	2h

研究领域	Neuro science;
检测原理	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig OX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig OX in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O77668

标准曲线图

【仅供科学研究使用】

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