猴肿瘤坏死因子配体超家族成员13(TNFSF13)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Simian TNFSF13(Tumor Necrosis Factor Ligand Superfamily, Member 13) ELISA Kit","alternative_names":"CD256; APRIL; TALL2; TRDL1; ZTNF2; A Proliferation Inducing Ligand; TNF And APOL Related Leukocyte Expressed Ligand 2; TNF-Related Death Ligand 1","species":"Simian","assay_type":"Sandwich","sensitivity":"15.74 pg\/mL","standard":"3000 pg\/mL","detection_range":"46.88-3000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"CD & Adhesion molecule;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TNFSF13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TNFSF13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TNFSF13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TNFSF13 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11595-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11595-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11595",}