猴胃泌素(GT)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Simian GT(Gastrin) ELISA Kit","alternative_names":"GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III","species":"Simian","assay_type":"Sandwich","sensitivity":"4.9 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian GT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian GT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian GT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian GT in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11715-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11715-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11715",}