猪胃泌素抑制肽(GIP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Pig GIP(Gastric Inhibitory Polypeptide) ELISA Kit","alternative_names":"Incretin hormone; Glucose Dependent Insulinotropic Peptide","species":"Pig","assay_type":"Competitive Inhibition","sensitivity":"23.3 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Endocrinology;Gastroenterology;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig GIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig GIP in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11798-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11798-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11798",}