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人转化生长因子β1(TGFb1)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK1185MS
  • 价格:¥2,400.00 元
  • 点击:2325
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产品介绍

产品信息

英文名称Human TGFb1(Transforming Growth Factor Beta 1) Microsample ELISA Kit
别名TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Human
检测类型Sandwich
灵敏度6.1 pg/mL
检测范围15.63-1000 pg/mL
样本类型serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cytokine;Tumor immunity;Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P01137

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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