人上皮中性粒细胞激活肽78(ENA78)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human ENA78(Epithelial Neutrophil Activating Peptide 78) Microsample ELISA Kit","alternative_names":"CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78","species":"Human","assay_type":"Sandwich","sensitivity":"0.053 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ENA78. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENA78. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ENA78, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENA78 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P42830","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1277MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1277MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1277MS",}