小鼠单核细胞趋化蛋白5(MCP5)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse MCP5(Monocyte Chemotactic Protein 5) Microsample ELISA Kit","alternative_names":"CCL12; SCYA12; Chemokine C-C-Motif Ligand 12; MCP-1-related chemokine; Monocyte chemoattractant protein 5; Small-inducible cytokine A12","species":"Mouse","assay_type":"Sandwich","sensitivity":"5.3 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;Infection immunity;Human gene deletion;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MCP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MCP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MCP5 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q62401","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1378MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1378MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1378MS",}