大鼠骨成型蛋白受体1A(BMPR1A)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Rat BMPR1A(Bone Morphogenetic Protein Receptor 1A) Microsample ELISA Kit","alternative_names":"CD292; ACVRLK3; ALK3; SKR5; BMPR1-A; Activin A Receptor, Type Ii-Like Kinase 3; Activin Receptor-Like Kinase 3; Serine\/Threonine-Protein Kinase Receptor R5","species":"Rat","assay_type":"Sandwich","sensitivity":"0.25 ng\/mL","standard":"50 ng\/mL","detection_range":"0.79-50 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;CD & Adhesion molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMPR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMPR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMPR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMPR1A in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q78EA7","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1407MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1407MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1407MS",}