大鼠胶质细胞系来源神经营养因子(GDNF)酶联免疫吸附检测试剂盒

英文名称	Rat GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
别名	ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor

种属	Rat
检测类型	Sandwich
灵敏度	33 pg/mL
检测范围	78.13-5000 pg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Cytokine;Apoptosis;Neuro science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDNF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q07731

标准曲线图

【仅供科学研究使用】

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