人血管活性肠肽(VIP)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human VIP(Vasoactive Intestinal Peptide) Microsample ELISA Kit","alternative_names":"PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27","species":"Human","assay_type":"Sandwich","sensitivity":"2.65 pg\/mL","standard":"500 pg\/mL","detection_range":"7.82-500 pg\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Endocrinology;Gastroenterology;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VIP in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01282","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1453MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1453MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1453MS",}