人周期素依赖性激酶抑制因子2A(CDKN2A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human CDKN2A(Cyclin Dependent Kinase Inhibitor 2A) ELISA Kit","alternative_names":"P16; ARF; CDK4I; CMM2; INK4; INK4a; MLM; MTS1; TP16; P14; P14ARF; P16INK4a; P19; Melanoma P16; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1","species":"Human","assay_type":"Sandwich","sensitivity":"0.267 ng\/mL","standard":"40 ng\/mL","detection_range":"0.63-40 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;Developmental science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDKN2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDKN2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDKN2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDKN2A in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P42771","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1460-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1460-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1460",}