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人热休克蛋白40(HSP40)酶联免疫吸附检测试剂盒

  • 货号:ELK1491
  • 价格:¥2,600.00 元
  • 点击:1461

产品介绍

产品信息

英文名称Human HSP40(Heat Shock Protein 40) ELISA Kit
别名DnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.055 ng/mL
检测范围0.16-10 ng/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP40 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P25685

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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