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兔诱导型一氧化氮合成酶(NOS2/iNOS)酶联免疫吸附检测试剂盒

  • 货号:ELK1505
  • 价格:¥2,800.00 元
  • 点击:1533

产品介绍

产品信息

英文名称Rabbit NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
别名NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rabbit
检测类型Sandwich
灵敏度0.064 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号O19114

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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