大鼠β-骨胶原交联(bCTx)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat bCTx(Beta-Crosslaps) ELISA Kit","alternative_names":"Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction","species":"Rat","assay_type":"Competitive Inhibition","sensitivity":"39.1 pg\/mL","standard":"8000 pg\/mL","detection_range":"125-8000 pg\/mL","sample_type":"serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Bone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1546-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1546-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1546",}