小鼠磷酸肌醇-3-激酶2α肽(PIK3C2a)酶联免疫吸附检测试剂盒

英文名称	Mouse PIK3C2a(Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide) ELISA Kit
别名	PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

种属	Mouse
检测类型	Sandwich
灵敏度	0.059 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Signal transduction;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIK3C2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIK3C2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIK3C2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIK3C2a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q61194

标准曲线图

【仅供科学研究使用】

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