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大鼠90kDa热休克蛋白β1(HSP90b1)酶联免疫吸附检测试剂盒

  • 货号:ELK1610
  • 价格:¥2,600.00 元
  • 点击:871

产品介绍

产品信息

英文名称Rat HSP90b1(Heat Shock Protein 90kDa Beta 1) ELISA Kit
别名HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1; 94 kDa glucose-regulated protein; Endoplasmin

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度0.05 ng/mL
检测范围0.16-10 ng/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;Immune molecule;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSP90b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSP90b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSP90b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSP90b1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q66HD0

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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