大鼠神经型一氧化氮合酶(NOS1)酶联免疫吸附检测试剂盒

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• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat NOS1(Nitric Oxide Synthase 1, Neuronal) ELISA Kit","alternative_names":"NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1","species":"Rat","assay_type":"Sandwich","sensitivity":"0.053 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Enzyme & Kinase;Metabolic pathway;Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P29476","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1620-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1620-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1620",}