{"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human NMB(Neuromedin B) ELISA Kit","alternative_names":"NM-B","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"48.1 pg\/mL","standard":"10000 pg\/mL","detection_range":"156.25-10000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2h","research_area":"Signal transduction;Endocrinology;Neuro science;Gastroenterology;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NMB in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P08949","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1629-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1629-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1629",}