人血小板膜糖蛋白Ⅱb/Ⅲa(GPIIb/IIIa)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human GPIIb\/IIIa(Glycoprotein IIb\/IIIa) ELISA Kit","alternative_names":"GP-IIb\/IIIa","species":"Human","assay_type":"Sandwich","sensitivity":"0.058 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"CD & Adhesion molecule;Metabolic pathway;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPIIb\/IIIa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPIIb\/IIIa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPIIb\/IIIa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPIIb\/IIIa in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1688-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1688-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1688",}