大鼠趋化因子C-X-C-基元配体16(CXCL16)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Rat CXCL16(Chemokine C-X-C-Motif Ligand 16) Microsample ELISA Kit","alternative_names":"SR-PSOX; CXCLG16; SRPSOX; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16","species":"Rat","assay_type":"Sandwich","sensitivity":"0.117 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Cytokine;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CXCL16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CXCL16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CXCL16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CXCL16 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q6AXU5","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1706MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1706MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1706MS",}