{"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human POMC(Proopiomelanocortin) ELISA Kit","alternative_names":"POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"44 pg\/mL","standard":"10000 pg\/mL","detection_range":"156.25-10000 pg\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"2h","research_area":"Endocrinology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human POMC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POMC in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01189","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1875-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1875-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1875",}