人基质金属蛋白酶23B(MMP23B)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human MMP23B(Matrix Metalloproteinase 23B) Microsample ELISA Kit","alternative_names":"MMP22; MMP23-B; MIFR; MIFR-1; Matrix Metalloproteinase 22; Femalysin; Matrix Metalloproteinase In The Female Reproductive Tract","species":"Human","assay_type":"Sandwich","sensitivity":"29 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP23B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP23B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP23B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP23B in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1938MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1938MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1938MS",}