{"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human dSIP(Delta-Sleep Inducing Peptide) ELISA Kit","alternative_names":"DSIPI; TSC22D3; DIP; GILZ; TSC-22R; hDIP; TSC22 Domain Family Protein 3; Glucocorticoid-Induced Leucine Zipper; Delta Sleep Inducing Peptide,Immunoreactor","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"7.49 pg\/mL","standard":"1500 pg\/mL","detection_range":"23.44-1500 pg\/mL","sample_type":"Serum, plasma, other biological fluids","assay_length":"2h","research_area":"Reproductive science;Neuro science;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human dSIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dSIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dSIP in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1946-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1946-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1946",}