人转化生长因子β刺激蛋白22(TSC22)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human TSC22(Transforming Growth Factor Beta Stimulated Protein Clone 22) Microsample ELISA Kit","alternative_names":"TSC22D1; TGFB1I4; TSC22; hucep-2; Cerebral protein 2; Transforming Growth Factor Beta 1 Induced Transcript 4; TSC22 Domain Family,Member 1","species":"Human","assay_type":"Sandwich","sensitivity":"0.104 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSC22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSC22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSC22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSC22 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2131MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2131MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2131MS",}