人免疫球蛋白G2(IgG2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human IgG2(Immunoglobulin G2) ELISA Kit","alternative_names":"IGHG2; Ig Gamma-2 Chain C Region; Immunoglobulin Heavy Constant Gamma 2; G2m Marker; Immunoglobulin Gm2","species":"Human","assay_type":"Sandwich","sensitivity":"0.51 µg\/mL","standard":"100 µg\/mL","detection_range":"1.57-100 µg\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P01859","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2143-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2143-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2143",}