人白介素2受体a链(IL-2Ra/CD25)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human IL-2sRa\/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit","alternative_names":"IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha","species":"Human","assay_type":"Sandwich","sensitivity":"6.5 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa\/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa\/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa\/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa\/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2178MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2178MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2178MS",}