人基质金属蛋白酶13(MMP13)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human MMP13(Matrix Metalloproteinase 13) ELISA Kit","alternative_names":"CLG3; Collagenase 3","species":"Human","assay_type":"Sandwich","sensitivity":"0.108 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;Tumor immunity;Bone metabolism;Rheumatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP13 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P45452","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2185-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2185-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2185",}