人神经调节素1(NRG1)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human NRG1(Neuregulin 1) Microsample ELISA Kit","alternative_names":"ARIA; GGF; HGL; HRG; HRGA; NDF; SMDF; Acetylcholine receptor-inducing activity; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor","species":"Human","assay_type":"Sandwich","sensitivity":"0.051 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NRG1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q02297","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2232MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2232MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2232MS",}