人髓鞘关联糖蛋白(MAG)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human MAG(Myelin Associated Glycoprotein) Microsample ELISA Kit","alternative_names":"GMA; S-MAG; Siglec-4; SIGLEC4A; Sialic Acid Binding Ig-Like Lectin 4A","species":"Human","assay_type":"Sandwich","sensitivity":"0.333 ng\/mL","standard":"50 ng\/mL","detection_range":"0.78-50 ng\/mL","sample_type":"tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Neuro science;Autoimmunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAG in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P20916","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2365MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2365MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2365MS",}